聚(4-乙烯基吡啶)-b-聚环氧乙烷-b-聚(4-乙烯基吡啶)的合成及其用于毛细管电泳分离碱性蛋白质

Synthesis of Poly(4-vinylpyridine)-b-Poly(ethylene oxide)-b-Poly(4-vinylpyridine) and Its Application for Separation of Alkaline Protein by Capillary Electrophoresis

用原子转移自由基聚合(ATRP)法合成了结构可控的三嵌段共聚物聚(4-乙烯基吡啶)-b-聚环氧乙烷-b-聚(4-乙烯基吡啶)(P4VP-b-PEO-b-P4VP)。用核磁共振氢谱和凝胶渗透色谱对该共聚物进行了表征;将该共聚物作为毛细管物理吸附涂层,用毛细管电泳对碱性混合蛋白质进行了分离。结果表明:蛋白质的分离效率随着P4VP嵌段长度的增加而提高。

A well-defined triblock copolymer,poly（4-vinylpyridine）-b-poly（ethylene oxide）-b-poly（4-（vinylpyridine））（P4VP-b-PEO-b-P4VP）,was synthesized by atom transfer radical polymerization（ATRP）,and characterized by ~1H-NMR and gel permeation chromatography.P4VP-b-PEO-b-P4VP was successfully used as physical coating in the capillary for alkaline protein separation by capillary electrophoresis（CE）.P4VP-b-PEO-b-P4VP with higher molecular mass of P4VP block achieved higher separation（efficiency） of the three alkaline proteins.