期刊文献+

地塞米松对胃癌细胞核骨架—中间纤维系统的影响 被引量:1

Effect of dexamethasone on the nuclear matrix\| intermediate filament system in gastric carcinoma cells
下载PDF
导出
摘要 本文应用选择性抽提整装电镜观察,研究地塞米松诱导人胃癌 M Gc803 细胞分化过程中细胞核骨架中间纤维系统的构型变化。 M Gc803 细胞核骨架纤维和中间纤维数量较少、分布不均匀,核纤层为厚薄不一结构,与两类纤维联系不密切。经 10μg/m l Dex 处理后,细胞核骨架纤维和中间纤维数量增多、结构层次丰富、单丝成份多,分布均匀并相互交织成规则网络,两类纤维通过薄层均一的核纤层发生密切联系,形成贯穿整个细胞核质区域的完整体系。结果表明, Dex 诱导处理细胞的核骨架中间纤维系统产生了与正常细胞相似的恢复性改变,这种变化是癌细胞恶性表型逆转的重要形态和功能表现。 The configurational changes of the nuclear matrix\|intermediate filament (NM\|IF) system in human gastric adenocarcinoma cell line MGc80\|3 after being induced by dexamethasone (Dex) had been studied by selective extraction\|whole mount electron microscopy.It was revealed by electron microscopy that,the NM and IF filaments in MGc80\|3 cells was relatively few and scattered,not well\|distributed and arranged irregularly.The nuclear lamina was nonuniformly thick and compact,connected to NM and IF filaments relaxedly.But in the cells induced by 10μg/ml Dex,the two kinds of filament were plentiful and well\|distributed,differed in slender and thick form and interweaved into a regular network.The NM and IF filaments connected closely by thin and compact fiber\|like lamina,and form a regular network through the whole cell region.It demonstrated that,the NM\|IF system in MGc80\|3 cell had been undergone a restorational change similar to that of normal cells after Dex treatment,and this alternation is an important morphological and functional expression to the maligant phenotypic reversion of cancer cells. 
作者 李祺福
出处 《电子显微学报》 CAS CSCD 1999年第4期410-413,共4页 Journal of Chinese Electron Microscopy Society
关键词 核骨架 中间纤维系统 胃癌细胞 地塞米松 nuclear matrix\|intermediate filament gastric carcinoma cell dexamethasone induced differentiation
  • 相关文献

参考文献3

  • 1陈枫,翟中和.细胞核骨架[J].细胞生物学杂志,1989,11(2):49-53. 被引量:8
  • 2蒋栋良.-[J].国外医学(分子生物学),1989,11(1):18-21.
  • 3蒋栋良,国外医学.分子生物学分册,1989年,11卷,1期,18页

二级参考文献2

  • 1Zhai Zhonghe,Sci Chin B,1988年,10卷,1052页
  • 2Zhai Zhonghe,Beijing Symposium on Electron Microscopy,1986年

共引文献7

同被引文献14

  • 1Ll Qi-Fu(Laboratory of Cell Biology, XiamenUniversity, Xiamen 361O05, Fuian Province, China).Effect of retinoic acid on the changes of nuclear matrix-intermediate filament system in gastric carcinoma cells[J].World Journal of Gastroenterology,1999,5(5):417-420. 被引量:17
  • 2汪玲,黄宗平,李祺福.HMBA对人胃腺癌细胞NM-IF系统形态的影响[J].电子显微学报,1996,15(1):21-24. 被引量:1
  • 3Getzenberg R H,Konety B R,Oeler T A,et al.Bladder cancer-associated nuclear protein[J].Cancer Res.,1996,56:1 690-1 694.
  • 4Konety B R,Nguyen T T,Dhir R,et al.Detection of bladder cancer using a novel nuclear matrix protein,BLCA-4[J].Clin.Cancer Res.,2000,6:2 618-2 625.
  • 5Konety B R,Nangia A K,Nguyen T S,et al,Identification of nuclear matrix protein alteratrions associated with renal cell carcinoma[J].J.Urol,1998,159(4):1 359-11 363.
  • 6Brünagel G,Shah U,Schoen R E,et al.Identification of calreticulin as a nuclear matrix protein associated with human colon cancer[J].J.Cell.Biochem,2003,89:238-243.
  • 7Konety B R,Getzenberg R H.Nuclear structural proteins as biomarkers of cancer[J].Journal of Cellular Supplements,1999,32/33:183-191.
  • 8Berezney R,Mortillaro M J,Ma H,et al.The nuclear Matrix:A structural milieu for nuclear genomic function[J].Int.Rev.Cytol,1995,162A:1-65.
  • 9Bidwell J P,Alvarez M,Feister H,et al.Nuclear matrix proteins and osteoblast gene expression[J].J.Bone.Miner.Res.,1998,13(2):155-167.
  • 10S'iakste N I,S'iakste T G.Transcription factors and the nuclear matrix[J].Mol.Biol.(Mosk),2001,35(5):739-749.

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部