姜黄中姜黄素的提取与分离工艺研究

Study on extraction and separation of curcumin from turmeric

采用乙醇加热回流和超声两种方法提取与分离姜黄素,采用氯仿∶乙酸乙酯∶甲醇=38∶1∶1和75∶1∶1作为梯度洗脱剂的硅胶柱层析分离姜黄提取液,采用薄层法监测分离。结果表明,乙醇加热回流提取法较佳,提取条件为浓度65%乙醇,提取2次,固液比为1∶10,提取时间2 h,此工艺简单可行。

The heating backflow method and the ultrasonic extraction were all investigated to extract curcumin from turmeric.The coarse extraction liquid was separated by silica gel column chromatography and the eluant of chloroform,ethyl acetate and methanol was 38 ∶1 ∶1 and 75 ∶1 ∶1,the separation results were tested by thin layer chromatography.The orthogonal experimental results in heating backflow showed that the extraction of curcumin was obviously improved under the conditions of extraction twice with 65% ethanol for 2 h every time and the ratio of solid to liquid was 1 ∶10.The process was simple and feasible.