蓖麻基润滑油降解菌群的构建及对土壤的修复作用

Study on construction of degradable microorganisms for lubricant-castor and its bioremediation to soil

以添加蓖麻基润滑油的土壤为模拟实验对象,从大港油田油井附近受污染的土壤和污水中筛选出优势降解菌株,构建高效降解菌群,确定了BM-1+BM-2+BM-4+BM-5菌株为最佳组合.将菌群投加到模拟被润滑油污染的土壤中进行生物修复,以土壤生物活性及土壤呼吸强度作为考核指标,40 d后土壤含油量从89 g·kg 1下降到24 g·kg 1,降解率达73.03%,比自然条件下降解率提高34.83%.结果表明,投加该菌群的污染土壤生物修复能力强于未投加菌群的污染土壤;蓖麻基润滑油为可生物降解型油品.

Through the experiments of lubricant-castor degradation,a series of microorganisms were screened to con-struct a high-effective microbial consortium,and confirmed the optimal combination was BM-1＋BM-2＋BM-4＋BM-5.Adding it to the soil polluted by lubricant-castor for biological repairing,with soil respiration intensity and biological ac-tivity of change as indicators of bioremediation,the results showed that during the process of 40-day bioremediation,the degradation rate was improved by 34.83% compared with that in nature condition,plus bacteria bioremediation of soil strength significantly larger than that without bacteria with soil samples,lubricant-castor was biodegradable oil.