纤维素三苯甲酸酯薄层板的制备及对几种常用药物的拆分研究

Preparation of Cellulose Tribenzoyl for TLC and Research on the Resolution of Several Commonly Used Drugs

采用微晶纤维素为原料与苯甲酰氯在超声条件下合成纤维素三苯甲酸酯(CTB),将所得产物用红外光谱与元素分析进行表征;按一定的的比例将CTB与微晶纤维素混合制备手性薄层板;采用合适的展开系统及显色方法,对氧氟沙星、普萘洛尔、苯丙氨酸、N-甲基-β-羟基苯乙胺、阿托品等五种手性药物进行拆分,并对拆分所得的对映体经比旋度测定及熔点、红外、氢谱等结构确证,均与文献报道相符。

Tribenzoyl-cellulose（CTB） was ultrasonically prepared by reaction of microcrystalline cellulose with benzoyl chloride and characterized by IR spectrometry and element analysis.The mixture of CTB and microcrystalline cellulose in certain propotion was used as chiral stationary phase（CSP） of thin-layer chromatography.Optimized of mobile phases and visualization,five chrial drugs were separated on the CSP,such as ofloxacin,propranolol,phenylalanine,N-methyl-β-hydroxyl-phenylethylamine and atropine.Enantiomers obtained were characterized by specific rotation,melting point,IR and 1H-NMR spectrometry and these datas were consistent with the reported.