径向电场调制毛细管电泳法用于蛋白质分离

Capillary Electrophoresis of Proteins Under the Control of Radial Electric Field

利用自制的双向电场控制毛细管电泳新系统， 考察了蛋白质的分离情况． 结果发现， 在低ｐＨ 值下， 通过施加径向电场， 不仅可改变电渗流的大小和方向， 而且能抑制蛋白质的吸附， 进而实现对蛋白质分离效率和分离速度的调控． 研究结果表明， 可通过物理化学方法实现毛细管电泳的动态或随机调控，

Separation of basic proteins was performed using a homemade electric field modulated capillary electrophoretic system, which can offer both radial and axial electric fields with only one high voltage power supply. The most interesting phenomenon observed is that the adsorption of protein molecules onto the tubing wall can be regulated by the application of a radial voltage(potential at the outer wall minus that at the inner wall): band broadening decreases with the increase of the radial voltage. This implies that it may be possible to dynamically control the adsorption of protein molecules on silica surface. In addition, the radial voltage is also able to adjust the resolution of the solutes due to the change of electroosmosis.