摘要
The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.
The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4_100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第10期1578-1580,共3页
Chemical Journal of Chinese Universities
基金
华东理工大学生物反应器工程国家重点实验室开放基金
关键词
亚麻酸油醇酯
合成
酯蜡
脂肪酶
酯化
催化剂
Ester wax, Lipase, Esterification reaction, Microaqueous system
