OAP H_2O_2HRP伏安酶联免疫分析新体系测定人血清总甲状腺素

OAP H 2O 2 HRP Voltammetric Enzyme linked Immunoassay for Trace Thyroxine in Human Serum

提出邻氨基酚（ＯＡＰ）Ｈ２Ｏ２辣根过氧化物酶（ＨＲＰ） 伏安酶联免疫分析新体系并用于人血清中总甲状腺素的测定。本方法以线性扫描二阶导数伏安法检测ＨＲＰ催化Ｈ２Ｏ２氧化ＯＡＰ的产物， 用于游离ＨＲＰ和ＨＲＰ标记物的测定，灵敏度均高于经典的ＥＬＩＳＡ显色光度法。测定游离ＨＲＰ的线性范围为１．０×１０－ １２～４．０×１０－ ９ ｇ／ｍ Ｌ， 检测限达６．０×１０－ １３ ｇ／ｍ Ｌ。本法对总甲状腺素测定的线性范围为１．０～３２０ ｎｇ／ｍ Ｌ。用所建立的方法对人血清样品进行了测定，并与现行的ＥＬＩＳＡ显色光度法进行对照，二者相关性很好。

A voltammetric enzyme linked immunoassay method based on the new system of o aminophenol ( OAP ) H 2O 2 horseradish peroxidase ( HRP ) has been developed and used for the determination of HRP and thyroxine(T 4). HRP or labelled HRP catalyzes the oxidation reaction of OAP by H 2O 2, the product of which produces a sensitive voltammetric peak at a potential of 0.43 V ( vs. SCE ) in Britton Robinson buffer solution. By using this voltammetric peak, HRP can be measured with a detection limit of 6.0×10 -13 g/mL and a linear range of 1.0×10 -12 ～4.0×10 -9 g/mL. The detection limit for thyroxine(T 4) is 1.0 ng/mL and the linear range 1.0～320 ng/mL. Some human serum samples have been analyzed and satisfactory results have been obtained