摘要
研究了Ｃｏ（Ⅱ）在碱性介质中催化过氧化氢氧化邻硝基苯基荧光酮（ｏＮＰＦ）这一新指示反应及影响反应速度的因素，建立了测定痕量Ｃｏ（Ⅱ）的新方法。灵敏度为４．３×１０－ １０ｇ／Ｌ；测定范围为０～０．５ｎｇ／２５ｍ Ｌ。方法简便快速，用于药物维生素Ｂ１２中Ｃｏ（Ⅱ）含量的测定，样品分析结果良好。
In a basic medium, trace cobalt( Ⅱ ) can catalyze the decolorizing reaction resulting from the oxidation of o nitrophenyl fluorone( o NPF)by H 2O 2.A new catalytic spectrophotometric method for determining trace Co(Ⅱ) was established. The factors affecting the reaction speed were investigated. The sensitivity of the method is 4.3×10 -10 g/L ,the linear response is in the range of 0～0.5ng/25mL.The method has been used to determine trace Co(Ⅱ) in vitamin B 12 samples. The average recoveries were in the range of 97.6～101.1%.The relative standard deviation was 2.48～2.62% for six replicate determinations. The method is simple and accurate.
出处
《分析试验室》
CAS
CSCD
1999年第5期59-62,共4页
Chinese Journal of Analysis Laboratory
关键词
催化光度法
钴
NPF
测定
Catalytic spectrophotometry
cobalt
o nitrophenylfluorone( o NPF)
