用四磺基铝酞菁直接测定血清中白蛋白

Determination of Human Serum Albumin by Aluminium Tetrasulponated Phthalocyanine

以红区荧光染料四磺基铝酞菁(AlS_4Pc)为探针建立了荧光猝灭法测定白蛋白的新方法.考察了各种影响因素,在最佳实验条件下,该方法的线性区间0.10～4.5mg/L,检测限为40μg/L.将该方法用于人血清样品中白蛋白的直接测定并与临床方法(溴甲酚绿法)比较,二者符合良好.

Based on the fluorescence quenching of aluminium tetrasulphonated phthalocyanine (AlS4Pc), a red-region fluorescent dye, reacted with albumin, a new method was developed for the determination of human serum albumin(HSA). Under the experimental conditions, the linear range ofthis assay was 0. 10-4.5 mg/Land the detection limit was 40μg/L. The method has been applied to theanalysis of human serum samples and the results were in agreement well with those achieved by bromocresol green method, which indicated that the method was not only sensitive but also reliable.