复方天葡片抑制H_2O_2诱导小鼠淋巴细胞

Protective Effect of Compound Tianpupian(TPP) against H_2O_2-induced Apoptosis of Murine Splenic Lymphocytes

本研究旨在探讨复方天葡片(tianpupian,TPP)抑制过氧化氢(H2O2)诱导淋巴细胞凋亡的作用机制。采用MTT法观察TPP对H2O2导致的淋巴细胞死亡率的影响;应用流式细胞仪AnnexinV/PI双染法、JC-I线粒体膜电位及caspase3酶活性的荧光化学发光法检测TPP对H2O2诱导淋巴细胞凋亡的抑制作用。结果表明:TPP通过拮抗H2O2导致的线粒体膜电位下降,抑制caspase3酶活性而降低H2O2诱导的淋巴细胞凋亡率。结论TPP对H2O2诱导的淋巴细胞凋亡具有显著的抑制作用,其机制与线粒体膜电位下降及caspase3酶活性抑制有关。

The aim of this study was to explore the protective effect of compound tianpupian（TPP） against H2O2-induced the apoptosis of murine splenic lymphocytes and its machanism.The cell apoptosis rate was detected by MTT method;the cell apoptosis and mitochondrial membrance potential were detected by flow cytometry（FCM） with Annexi-V/ PI double staining and JC-1 staning method,respectively;and caspase 3 relative activity was determined by colorimetry.The results indicated that after treating with H2O2,the absorbance value of cultured lymphocytes and the red/green ratio of JC-1 were reduced,and the apoptotic rate and caspase 3 activity were increased,coculture of H2O2-treated cells with compound TPP increased the cell absorbance ratio and red/green rate of JC-1,while reduced the apoptosis rate and caspase 3 activity.It is concluded that compound TPP alleveates intracellular oxidative damages and dose-dependently inhibited apoptosis of murine splenic lymphocytes through reducing mitochondrial membrane potental and inhibiting caspase 3 activity.This suggests that compound TPP is a potential anti-apoptotic agent.