中国南京地区十个红细胞血型系统稀有抗原的大规模调查

A Large-scale Survey for Rare Blood Group Screening among Blood Donors in Chinese over Nanjing Area

本研究调查南京地区人群中稀有血型抗原的类型及分布情况,为稀有血型病人的临床用血和献血招募提供基础性资料。采用4M尿素对红细胞进行Kidd血型系统的Jk(a-b-)表型筛选;利用抗血清对H系统的H-、MNS系统的GPA-、Gerbich系统的GPC-、Ii系统的i+、Lutheran系统的Lub-表型进行筛选;对Kell系统的k-和Jsb-、Duffy系统的Fya-、Ok系统的Ok-、MNS系统的s-和Digeo系统的Dib-表型采用多重PCR的方法进行筛选。结果表明:在40337例标本中,筛选出2例Jk(a-b-)表型;在1 782例标本中,筛出Fy(a-b+)表型3例,尚未发现Fy(a-b-)的表型,也未发现Ok-、s-和Dib-表型。在2 002、2 003、2 084、10 189和10 004例标本中,未发现相应的GPA-、GPC-、Lub-、H-和i+稀有表型者。在2017例标本中,也未发现k-和Jsb-的表型存在。结论:南京地区人群中Jk(a-b-)表型频率为0.0049%,Fy(a-b+)表型频率为0.168%,在现有筛查人数中尚未发现所查其它血型系统稀有抗原的存在。

The purpose of this study was to investigate the distribution of 10 rare red blood groups in Chinese Nanjing population,so as to provide compatible rare blood to patients and to create a donor data bank.Jk（a-b-）（Kidd） phenotypes were detected by urea,while H-（H）,GPA-（MNS）,GPC-（Gerbich）,i＋（Ii） and Lub-（Lutheran） phenotypes were detected by monoclonal,polyclonal antibodies with U type 96 well microplate technology.The screening of Jsb-and k-（Kell）,Fya-（Duffy）,Ok-（Ok）,s-（MNS） and Dib-（Digeo） phenotypes were performed by polymerase chain reaction.The results showed that 2 Jk（a-b-） out of 40337 donation samples and 3 Fy（a-b＋）out of 1782 donation samples were found,while no other rare blood phenotypes（H-、GPA-、GPC-、Lub-、Ok-、s-、Jsb-、k-、Dib-and i＋） were detected.It is concluded that the frequencies of Jk（a-b-） and Fya（a-b＋）are 0.0049% and 0.168% respectively.No more rare blood phenotype was found in this screening.