套式PCR法检测输卵管妊娠者的沙眼衣原体感染研究

NestedPCR detection of chlamydia trachomatis infection in patients with tubal pregnancy.

目的：为探讨输卵管妊娠与生殖道沙眼衣原体的关系。方法：采集５７例输卵管妊娠者的宫颈分泌物、输卵管组织、胚胎组织，并采集３２例卵巢囊肿者的宫颈分泌物、输卵管组织和２９例早孕人流者的宫颈分泌物、胚胎组织。应用套式ＰＣＲ法检测以上标本中的沙眼衣原体ＤＮＡ。结果：输卵管妊娠者的宫颈分泌物阳性率高达４０．４％，明显高于卵巢囊肿者的１２．５％和早孕人流者的１３．７％（Ｐ均＜０．０５），ＲＲ为４．４９；输卵管妊娠者的输卵管组织阳性率为２４．６％，明显高于卵巢囊肿者的６．３％，ＲＲ为４．９０；输卵管妊娠者的胚胎组织阳性率为２６．３％，明显高于早孕人流胚胎组织的６．９％，ＲＲ为４．８０。结论：输卵管妊娠与生殖道的沙眼衣原体感染相关。

Objective:To investigate the vdationship between genital infections with chlamydia trachomatis (ct) and tubal pregnancy.Methods:57 patients with tubal pregnancy (case group),32 patients with ovarian cyst (control group A) and 29 women with early pregnancy(control group B) were investigated as a casecontrol study.NestedPCR technique was used to detect ctDNA in samples from the secretion of cervix,fallopian tube and felal tissue.Results:Case group was more likly to have detectable CtDNA(40.4%)from cervical secretion than control group A and B (pall<0.05),RR was 4.49.The positive rate of case group from fallopian tube was highter than ovarian cyst (p<0.05)and the positive rate from fetal tissue was higher than early pregnancy(p<0.05),RR was 4.90 and 4.80. Conclusion:There is a strong association between tubal pregnancy and genital infection with Ct.