豚鼠甲流H1N1感染模型的建立及激素甲强龙对感染干预作用的探讨

Establishment of a Guinea Pig Model of Influenza A (H1N1) Virus Infection and Effect of Methylprednisolone Intervention

目的建立豚鼠的甲流感染模型并评价激素甲强龙对感染的干预作用。方法将4～6周龄雌性SPF级豚鼠(200～300)g分为4组:正常对照组,模型组,甲强龙1组和甲强龙2组,除对照组外,豚鼠经乙醚麻醉后进行滴鼻接种A/California/7/2009(CA7)病毒,分别于攻毒后3 d和5 d给予激素甲强龙,大剂量3 d后1/4剂量给3 d,检测豚鼠感染的多项指标,观察期为14 d。结果成功建立了豚鼠的甲流感模型;甲强龙1组豚鼠肺部炎症减轻较模型组和甲强龙2组明显,存活率较这两组均降低,甲强龙2组肺部炎症较模型组减轻,而较甲强龙1组重,豚鼠的存活率较这两组均无差异。结论豚鼠能感染A/California/7/2009(CA7)病毒,感染后5 d比感染后3 d开始给予激素的干预效果好。

Objective To establish a guinea pig model of influenza A/California/7/2009（CA7）virus infection and evaluate the intervention with hormone methylprednisolone for viral pneumonia due to this infection.Methods Female Hartley strain guinea pigs（200-300 g） were divided into four groups： control,model,methylprednisolone group 1 and methylprednisolone group 2.The animals were anesthetized by inhalation of ether and inoculated intranasally inoculated with 0.3 mL of virus CA7.Methylprednisolone was administered beginning at postinfection day 3 or 5,recommended dose lasting for 3 days and then quarter of recommended dose for another 3 days.The animals were observed daily for 14 days for clinical signs of infection and survival.Results The guinea pig model for A/California/7/2009（CA7）virus infection was established successfully.The lung inflammation of methylprednisolone group 1 was alleviated compared with that of both model group and methylprednisolone group 2,but the survival rate was lower compared with that of both two groups.The lung inflammation of methylprednisolone group 2 was alleviated in comparison with that in the model group but aggravated compared with that of methylprednisolone group 1,while the survival rate was not affected.Conclusion Guinea pigs can be infected with influenza A virus A/California/7/2009,and it is better to administer methylprednisolone on dpi 5 than dpi 3.