奥司他韦、利巴韦林和盐酸金刚乙胺对甲型H1N1流感病毒的抑制作用

Antiviral Activity of Oseltamivir Phosphate,Ribavirin and Rimantadine Hydrochloride on Influenza A/H1N1 Virus in Vitro

目的细胞水平测试奥司他韦、利巴韦林和盐酸金刚乙胺对甲型流感H1N1病毒的抑制或杀伤作用。方法通过在MDCK细胞系和甲型H1N1病毒株间建立药物剂量-效应关系确定导致细胞死亡的效力与抑制病毒复制的效力的比值(治疗指数),测试药物的抗病毒效果。结果奥司他韦、利巴韦林和盐酸金刚乙胺对MDCK细胞的半数中毒浓度分别为(1134.7±186.8)μg/mL、(742.0±76.9)μg/mL、(94.6±1.9)μg/mL,对甲型H1N1病毒的治疗指数(TI)分别为71.19、24.9和3.12。结论奥司他韦对甲型H1N1病毒抑制作用最强,利巴韦林其次,盐酸金刚乙胺对甲型H1N1病毒抑制效果较弱。

Objective To evaluate the antiviral activity of oseltamivir phosphate,ribavirin and rimantadine hydrochloride on influenza A（H1N1） in vitro.Methods Drugs diluted in FCS-free DMEM media supplemented with 2 μg/ mL TPCK-treated trypsin was applied to confluent MDCK cell monolayers for 1 h prior to addition of virus,at a challenge dose of one hundred times of the TCID50.The IC50 of drugs were calculated using neutral red assay after 3 days treatment at 34℃.Results The therapeutic index（TI） of oseltamivir phosphate against influenza A（H1N1） virus was 61.90,ribavirin was 42.86,and rimantadine hydrochloride was 6.22.Ubenimex was lower than 1.00.Conclusions Influenza virus is sensitive to oseltamivir phosphate and ribavirin.The antiviral effect of rimantadine hydrochloride is significantly lower than that of oseltamivir phosphate and ribavirin.