白藜芦醇对重症急性胰腺炎大鼠血小板功能的影响

Effects of resveratrol on platelet function in rats with severe acute pancreatitis

目的探讨白藜芦醇(Res)对重症急性胰腺炎(SAP)大鼠血小板功能的影响。方法制作SAP大鼠模型,将36只大鼠随机分为假手术组、SAP组和Res组,分别于造模后6 h、12 h测定血小板1 min聚集率(PAR-1)、最大聚集率(PAR-m)、血小板粘附率(PAR)及血浆血小板α颗粒膜蛋白水平(GMP-140)、血栓素B2(TXB2)水平。结果 SAP组大鼠血小板1 min聚集率、最大聚集率、粘附率及血浆GMP-140、TXB2水平显著高于假手术组(P<0.05)及Res组,Res组血小板1 min聚集率及血浆GMP-140、TXB2水平均低于SAP组(P<0.05)及假手术组(P<0.05)。结论 SAP大鼠血小板聚集性、粘附性、血浆GMP-140和TXB2水平均显著升高,血液处于高凝状态。Res可部分降低血小板活化程度,改善血小板的聚集、粘附和释放功能。

Objective To investigate the effects of resveratrol（Res） on platelet function in rats with severe acute pancreatitis（SAP）.Methods SAP was induced in rats.Rats were divided into 3 groups（12 rats in each group）： Sham group,SAP group and Res group.Indices of platelet function including platelet aggregation rate in first minute（PAR-1）,maximal platelet aggregation rate（PAR-m）,platelet adhesion rate（PAR） and the levels of GMP-140,TXB2 in plasma were determined after model establishment 6 h,12 h in above-mentioned three groups.Results The PAR-1,PAR-m,PAR and the levels of GMP-140,TXB2 in plasma were significantly increased in rats after induction of SAP（P0.05）.In Res group the PAR-1 and the levels of GMP-140,TXB2 in plasma were obviously lower than that in SAP group and Sham group（P0.05）.Conclusion The ability of platelet adhesion and aggregation,the levels of GMP-140 and TXB2 in plasma significantly increase after SAP.Res can partially reduce the degree of platelet activation and improve the platelet function of aggregation,adhension and release.