TFF2在胃癌、癌旁及正常胃黏膜组织中的表达及其与血管生成的关系

Correlation between TFF2 expression and tumor angiogenesis in gastric carcinoma, adjacent carcinoma and normal gastric mucosa

目的:探讨三叶因子2(TFF2)、血管内皮生长因子(VEGF)和微血管密度(MVD)在胃癌发生、发展、浸润和转移中的作用.方法:选取广西医科大学第一附属医院2008-01/2009-06接受胃大部切除术的胃癌标本50例,采用SP免疫组织化学方法检测30例正常胃黏膜组织、50例癌旁组织和50例胃癌组织中TFF2、VEGF和MVD的表达情况.结果:正常胃黏膜组织→癌旁组织→胃癌组织中,TFF2表达呈逐渐减弱趋势(165.80±16.42,184.44±19.02,206.79±17.62,均P<0.01),TFF2的表达与肿瘤的分化程度和淋巴结转移有关(均P<0.01),而VEGF的表达和MVD值呈逐渐上升趋势(36.7%,42.0%,72.6%;26.35±4.54,30.78±5.64,40.13±6.92,均P<0.01),两者表达与肿瘤的分化程度、浸润深度和淋巴结转移有关(均P<0.01).TFF2与MVD的表达呈负相关(r=-0.781,P<0.01).结论:TFF2作为一种胃癌的抑制因子,在胃癌发展过程中表达逐渐减弱,对胃癌的抑制作用降低,同时一些促进肿瘤浸润转移的因子如VEGF、MVD表达水平逐渐增强,促进了肿瘤的发展转移.

AIM： To investigate the correlation among the expression of trefoil factor 2 （TFF2） and vascular endothelial growth factor （VEGF） and microves- sel density （MVD） in gastric carcinoma. METHODS： The expression of TFF2 and VEGF and MVD were determined by immunohisto- chemistry in 50 gastric carcinoma specimens, 50 tumor-adjacent gastric mucosal specimens, and 30 normal gastric mucosa specimens. RESULTS： The expression level of TFF2 was highest in normal gastric mucosa, followed by tumor-adjacent gastric mucosa and gastric carcinoma （165.80 ± 16.42, 184.44 ± 19.02, 206.79 ± 17.62, all P 〈 0.01）. The expression of TFF2 in gastric cancer was significantly associated with the degree of tumor differentiation and lymph node metastasis （both P 〈 0.01）. The expression level of VEGF and MVD were lowest in normal gastric mucosa, followed by tumor-adjacent gastric mu- cosa and gastric carcinoma （36.7%, 42.0%, 72.6%; 26.35 ± 4.54, 30.78 ± 5.64, 40.13 ± 6.92, all P 〈 0.01）. The expression of VEGF and MVD in gastric cancer were associated with tumor differentiation, invasion and lymph node metastasis （all P 〈 0.01）. There is a negative correlation between the expression of TFF2 and MVD （r = -0.781, P 〈 0.01）. CONCLUSION： TFF2 may be a negative regulatory factor of gastric carcinogenesis. The expression of TFF2 is down-regulated during the development of gastric cancer, while that of VEGF and MVD are up-regulated during this process.