摘要
目的 用纤维蛋白原转化成的纤维蛋白刺激培养的血管平滑肌细胞,通过细胞计数和3H-胸腺嘧啶参入的方法,测定平滑肌细胞的增殖情况,并用 Northern 杂交的方法观测其c-fos、c-m ycm RNA水平。结果 纤维蛋白可明显促进平滑肌细胞的增殖,其作用呈浓度依赖关系。静止状态细胞,c-fos、c-m yc 的m RNA 呈低表达,纤维蛋白刺激后,二者表达均明显增加。结论 纤维蛋白刺激平滑肌细胞的增殖与促进c-fos、c-m
In this study, we investigated the effects of fibrin (Fb) converted from different concentrations of fibrinogen on cell counts, 3H thymidine incorporation, and c myc, c fos gene expression of cultured vascular smooth muscle cells (VSMC) The results showed the cell counts and 3H thymidine incorporation of VSMC after the stimulation of Fb increased in dose dependent manner The Nothern Blot results revealed that Fb could significantly increase the c fos and c myc mRNA expression compared with control group but no signigicant difference among the three different treatment groups Our study suggests that Fb could stimulate the proliferation of VSMC and this response involves the expression of c fos and c myc genes
出处
《中国介入心脏病学杂志》
1999年第3期137-139,共3页
Chinese Journal of Interventional Cardiology