冻干人尿中汞成分分析标准物质的研制

Development of the certified reference material of mercury in lyophilized human urine

目的研制冻干人尿中汞成分分析标准物质。方法冻干尿由正常人尿经过滤、加标、混匀、分装、冷冻干燥、辐射灭菌制备而成，使用原子荧光测定方法对其均匀性和稳定性进行检验，由3家实验室用准确可靠的方法确定标准值，并对尿中汞研制物的不确定度进行分析。结果本研制物均匀性良好，-20℃保存可稳定18个月，2个水平的量值及不确定度分别为（35．6±2．1）和（50．5±3．0）μg/L。结论尿中汞成分分析标准物质各项指标符合《国家一级标准物质研制规范》的要求，可用于实验室检测的质量控制。

Objective To develop the certified reference material of mercury in lyophilized human urine. Methods Human urine samples from normal level mercury districts were filtered, homogenized, dispensed, lyophilized and radio-sterilized. Homogeneity test, stability inspection and certification were conducted using a atom fluorescence spectrophotometric method. The physical and chemical stability of the certified reference material were assessed for 18 months. The certified values are based on analysis made by three independent laboratories. Results The certified values are as follows： low level was （35.6±2.1） μg/L, high level was （50.5±3.0） μg/L. Conclusion The certified reference material of mercury in lyophilized human urine in this research reached the national certified reference material requirements and could be used for the quality control.