尿中四氢呋喃的顶空固相微萃取-气相色谱测定法

Determination of tetrahydrofuran in urine by headspace solid-phase microextraetion and gas chromatography

目的建立测定尿中四氢呋喃的顶空固相微萃取一气相色谱法。方法以顶空固相微萃取技术处理尿样，用带氢焰离子化检测器的气相色谱仪测定尿中的四氢呋喃。通过对萃取头类型、萃取时间和温度、解吸温度和时间等影响萃取效率的因素的研究，获得了优化的试验条件。结果在优化条件下，方法的线性较好，样品的加标回收率为93．0％～100．8％，相对标准偏差为0．96％～3．85％，检出限可以达到0．5μg／L。结论该方法适用于尿中四氢呋喃的测定。

Objective Headspace solid-phase microextraction （HS-SPME） was used pre-concentration procedure for the determination of tetrahydrofuran in urine by gas chromatography with hydrogen flame detector. Methods Several parameters controlling SPME was studied and optimised： SPME fiber, extraction time and extraction temperature, desorption time and desorption temperature. Results Under optimal conditions, the correlation coefficient was 0.9998 and good recoveries （range from 93.0%-100.8%） were achieved, the detection limit was 0.5μg/L. Conclusion The method can be applied to the determination of trace amount of tetrahydrofuran in urine.