印楝油两性不育灭鼠颗粒制剂对高原鼠兔抗生育作用的研究

Studies on the anti-fertility effect of neem oil granules for rodents' ambisexual sterility on Ochotona curzoniae

为筛选印楝油两性不育灭鼠颗粒制剂的最优主药量,并探究其对高原鼠兔的抗生育作用,将主药浓度为300g/kg和400g/kg的印楝油两性不育灭鼠颗粒制剂分别进行摄食系数的研究,筛选出适口性较好的制剂,进一步研究适口性较好的制剂对高原鼠兔怀孕率和生殖器官的影响,并用免疫组织化学法检测输卵管和子宫的细胞凋亡情况。结果显示,主药浓度为300g/kg、400g/kg的颗粒制剂对高原鼠兔的摄食系数分别为0.97和1.87,二者之间差异显著(P<0.05);主药浓度为400g/kg的印楝油颗粒制剂对高原鼠兔的抗生育率为70%,对睾丸、附睾、子宫、输卵管和卵巢均有明显的病变影响,并且促进子宫细胞凋亡。结果表明,主药浓度为400g/kg的印楝油两性不育灭鼠颗粒制剂适口性良好,对高原鼠兔有良好的抗生育作用,有望用于草原鼠害的防制。

In order to choose the optimal dose of neem oil granules and study its anti-fertility action on Ochotona curzoniae,ingestion coefficients were observed at concentration of 300 g/kg or 400 g/kg of neem oil granules for rodents＇ambisexual sterility,respectively,and the apoptotic cells of oviduct and uterine were measured by immunohistochemical method.In result,the ingestion coefficients at concentration of 300 g/kg and 400 g/kg neem oil granules for rodents＇ambisexual sterility were 0.97 and 1.87 respectively,and the difference was significant compared with the normal control（P0.05）.The anti-fertility rate was 70% on O.curzoniae at 400 g/kg neem oil granules for rodents＇ambisexual sterility,and the dosage caused serious damages on testicle,epididymal duct,hystera,oviduct and ovary,and promoted apoptosis in uterine cells.In conclusion,the 400 g/kg of principle agent concentration of neem oil granules for rodents＇ ambisexual sterility had good palatability and significant anti-fertility effect,which has implications for the control of rodents＇ on grassland.