PGE_2,mPGES-1和HBx在肝细胞癌的表达及相关性

Correlation between hepatocellular carcinoma and the expressions of prostaglandin E_2,mPGES-1 and HBx

目的研究肝细胞癌(HCC)患者的前列腺素E2(PGE2)、微粒体型前列腺素E2合成酶-1(mPGES-1)与乙型肝炎病毒X蛋白(HBx)的表达情况、相关性和临床意义。方法采用酶联免疫法检测65例HCC患者外周血和癌组织的PGE2水平,用免疫组织化学法检测癌组织及癌旁肝组织mPGES-1和HBx的表达,并分析这些指标与患者的临床病理特征的关系。结果肝癌组织PGE2含量明显高于癌旁肝组织,分别为(9.10±2.03)ng/g和(2.78±0.19)ng/g;肝癌患者外周血中PGE2含量为(128.74±14.44)pg/ml,与肝癌组织中PGE2含量呈正相关(r=0.862);HCC组织中HBx和mPGES-1的阳性表达率分别为86.2%和78.5%,而癌旁肝组织的HBx和mPGES-1阳性表达率分别为81.5%和46.2%,其中mPGES-1蛋白在肝癌组织及癌旁肝组织的阳性表达率差异有统计学意义(P<0.05)。HBx和mPGES-1的表达升高与肝癌分化程度和肝外是否转移相关(P<0.05);HBx的表达与mPGES-1的表达呈正相关(r=0.389);外周血PGE2水平和组织mPGES-1的表达呈正相关(r=0.526)。结论肝细胞癌与PGE2、HBx、mPGES-1表达相关;可以通过检测肝细胞癌患者外周血的PGE2水平来辅助评估肝癌的复发转移;HBx和mPGES-1可作为肝癌的治疗靶点进行更深入的研究。

Objective To assess the correlation between prostaglandinE2 levels,the expression of hepatitis B virus X protein and microsomal PGE2 synthase-1 in patients with hepatocellular carcinoma,and to explore their clinical significance.Methods The PGE2 levels in peripheral blood and carcinoma tissues were measured by a kit of PGE2 ELISA.Expression of HBx and mPGES-1 proteins in HCC and adjacent tissues were detected by immunohistochemistry,then their correlation with clinicopathological features was assayed.Results The contents of PGE2 were significantly higher in the tissues of HCC（9.10±2.03） ng/g than those in tumor margins of excision（2.78±0.19） ng/g.The contents of PGE2 in peripheral blood were correlated with those in the HCC tissues（r=0.862）.Immunohistochemical staining results revealed that the positive expression of HBx and mPGES-1 were 86.2% and 78.5% in tumor tissues,81.5% and 46.2% in margins of excision,respectively.The expression of mPGES-1 in tumor tissues and margins of excision were statistically significant（P0.05）.The increased expression of HBx and mPGES-1 correlated with differentiation and hepatic metastasis.The expression of HBx and mPGES-1 in HCC tissues were in positive correlation（r=0.389）.The PGE2 levels in peripheral blood and expression of mPGES-1 in patients with HCC were enhanced,indicating a positive correlation of the levels in two samples（r=0.526）.Conclusion PGE2,HBx and mPGES-1 are involved in the processes of invasion and metastasis of HCC.PGE2 levels in peripheral blood might be used to evaluate the metastasis of HCC.HBx and mPGES-1 might be a potential new targets for HCC.