骨髓间充质干细胞对大鼠减体积肝移植术后肝细胞再生与修复的作用

The protective effects of bone-marrow-derived mesenchymal stem cells on hepatic regeneration and repair of rat liver graft after reduced-size liver transplantation

目的探讨骨髓间充质干细胞(BMSCs)对大鼠减体积肝移植术后肝脏再生与修复的影响。方法采用生化、免疫组化等方法检测术后不同时间的血清和肝脏标本。(1)取同周龄Wistar大鼠骨髓体外培养BMSCs,标记CSFE荧光后制备悬液;(2)建立大鼠50%减体积肝移植模型(供体鼠为SD大鼠,受体鼠为Wistar大鼠),分为实验组(取BMSCs悬液经门静脉植入)和对照组(取等量生理盐水经门静脉注射),观察大鼠生存状态,测量肝再生的相关指标。结果 (1)成功分离培养BMSCs;(2)术后2h,大鼠均自由活动饮水;(3)实验组肝组织中存在大量BMSCs,术后第2、3 d实验组肝体比显著高于对照组。病理显示:实验组肝细胞损伤较对照组轻微。结论植入BMSCs后,可以促进大鼠减体积肝移植术后肝细胞增殖和修复。

Objective To explore the effect of bone-marrow-derived mesenchymal stem cells（BMSCs） in promoting regeneration and repair of rat liver after reduced-size liver transplantation.Methods（1）BMSCS which were taken from the bone marrow of the Wistar rats were cultured in vitro,then marked by CSFE and prepared as suspension.（2）50% reduced-size liver transplantation models（SD donor to Wistar recipient） were established,which were divided into the experiment group（1ml BMSCs suspension was injected into portal vein） and the control group（1ml saline was injected into portal vein）.After operation,the living state of recipient was observed and the indicators about hepatic regeneration were detected through which serum and liver samples were collected and analyzed by biochemistry and immunohistochemistry respectively after transplantation.Results（1）BMSCs showed a high purity and homogeneity.（2）2 hours after liver transplantation,all rats were recovered moving freely and drinking water.（3）There were BMSCs in the liver graft.The ratio between liver weight and body weight in the experimental group was higher than the control group.Histopathology revealed that hepatocyte injury was minor in the experimental group than the control group.Conclusion BMSCs transfused into the liver graft can promote both regeneration and repair of liver cells after reduced-size liver transplantation.