TCF7L2基因rs7903146和rs11196218单核苷酸多态性与新诊断2型糖尿病患者早相胰岛素分泌的相关性分析

Correlation between single nucleotide polymorphisms of rs7903146 and rs11196218 at TCF7L2 gene and the early phase insulin secretion of newly diagnosed patients with type 2 diabetes

目的探讨重庆地区汉族人群中转录因子7类似物2(TCF7L2)基因rs7903146和rs11196218单核苷酸多态性(SNPs)与新诊断2型糖尿病(T2DM)早相胰岛素分泌的相关性。方法采用病例-对照研究方法,设新诊T2DM组(n=227)和正常对照组(n=152),采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术对rs7903146和rs11196218位点进行SNPs分型。结果新诊T2DM组和正常对照组rs7903146位点风险等位基因频率(MAF)分别为5.73%和4.28%,二者间差异无统计学意义。Logistic回归无论以共显性、显性、或是隐性模式分析,rs7903146和rs11196218位点的SNPs均与T2DM无显著相关性(P>0.05)。Rs7903146位点的SNPs与ΔI30/ΔG30相关(OR=1.012,95%CI 1.000～1.025,P=0.05),T风险等位基因携带者的ΔI30/ΔG30显著高于未携带者(P=0.0385)。结论尚未发现重庆地区汉族人群TCF7L2基因rs7903146和rs11196218位点SNPs与T2DM的相关性,但rs7903146位点的基因多态性可能与早相胰岛素分泌有关。

Objective To investigate the correlation between single nucleotide polymorphisms（SNPs） of rs7903146 and rs11196218 at TCF7L2 gene and the early phase insulin secretion of newly diagnosed patients with type 2 diabetes mellitus（T2DM）.Methods A case-control study was conducted in 227 newly diagnosed patients with T2DM（T2DM group） and 152 normal people（control group） from Chongqing area.SNPs of rs7903146 and rs11196218 were genotyped by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）.Results The minor allele frequencies（MAF） of rs7903146 in T2DM and control group were 5.73% and 4.28%,respectively,with no significant difference.Logistic regression analysis revealed that the SNPs of rs7903146 and rs11196218 were negatively associated with T2DM no matter with additive,dominant or recessive models（P〉0.05）.The SNPs of rs7903146 were associated with ΔI30/ΔG30（OR=1.012,95%CI 1.000-1.025,P=0.05）,the levels of ΔI30/ΔG30 in T-allele carriers were significantly higher than that in normal allele carriers（P=0.0385）.Conclusion There is no significant correlation between SNPs of rs7903146 and rs11196218 at TCF7L2 gene and newly diagnosed T2DM,but SNPs of rs7903146 may associate with the early phase insulin secretion.