丹参素异丙酯对慢性缺氧大鼠血压和左心室功能的影响

Effects of isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate at different concentrations on left ventricular functions of chronic hypoxic rats

目的:研究丹参素异丙酯对缺氧大鼠左心室功能的影响。方法:制备慢性缺氧大鼠模型,分别用大、小剂量的丹参素异丙酯灌胃,设立维拉帕米阳性对照组和正常对照组,采用大鼠心导管插入的方法,测定大鼠血流动力学和心功能指标,观察不同剂量丹参素异丙酯对慢性缺氧大鼠左室心功能的影响。结果:与正常组比较,单纯模型组的动脉收缩压(SBP)、动脉舒张压(DBP)、平均动脉压(MBP)、左室收缩压(LVSP)、最大上升速率(+dp/dtmax)和最大下降速率(-dp/dtmax)显著升高(P<0.05或P<0.01),T值(t-dp/dtmax)显著降低(P<0.05),HE染色显示,心肌细胞出现肌纤维断裂、溶解,细胞核固缩、溶解。与单纯模型组比较,小剂量组的DBP、MBP、心率(HR)、左室舒张末压(LVEDP)和-dp/dtmax显著降低(P<0.05或P<0.01),T值显著升高(P<0.05);大剂量组的SBP、DBP、MBP、HR、LVEDP、左室舒张压(LVDP)、+dp/dtmax和-dp/dtmax显著降低(P<0.05或P<0.01),T值显著升高(P<0.01);HE染色显示,大、小剂量组心肌细胞内糖元含量丰富,肌纤维排列相对规则,未见明显的肌纤维断裂、溶解及核分裂。与阳性对照组比较,小剂量组的DBP、MBP、LVSP、LVDP和T值显著升高(P<0.05或P<0.01),LV-EDP显著降低(P<0.05);大剂量组的DBP和T值均显著升高(P<0.05或P<0.01),LVDP、LVEDP和+dp/dtmax均显著降低(P<0.05或P<0.01)。与小剂量组比较,大剂量组的LVDP、LVEDP和+dp/dtmax均显著降低(P<0.01)。结论:丹参素异丙酯对慢性缺氧造成的大鼠血压升高、心率加快、心功能亢奋等有明显的抑制作用,因而对缺氧大鼠的心功能有明显的保护作用。

AIM： To observe the effects of isopropyl 3-（3,4-dihydroxyphenyl）-2-hydroxylpropanoate at different concentrations on the left ventricular function of chronic hypoxic rats.METHODS： Chronic hypoxic rats were equally divided into four groups： large-dose isopropyl 3-（3,4-dihydroxyphenyl）-2-hydroxylpropanoate group,small-dose isopropyl 3-（3,4-dihydroxyphenyl）-2-hydroxylpropanoate group,positive control group of verapamil and normal saline group.Homodynamic and heart function parameters were measured by intubating from left common carotid artery.RESULTS： Compared with those in normal group,SBP,DBP,MBP,LVSP,left ventricular ＋dp/dtmax and-dp/dtmax markedly increased（P〈0.05 or 0.01）,and t-dp/dtmax markedly decreased（P〈0.05） in model group,HE staining revealed disordered arrange of myocardial cell,fracture and dissolution of muscle fibers and nuclear fission in model group.Compared with those in the model group,DBP,MBP,HR,LVEDP and-dp/dtmax markedly decreased（P〈0.05 or 0.01）,and t-dp/dtmax markedly increased（P〈0.05） in the small-dose group,whereas SBP,DBP,MBP,HR,LVEDP,LVDP,＋dp/dtmax and-dp/dtmax markedly decreased（P〈0.05 or 0.01）,and t-dp/dtmax markedly increased（P〈0.01） in the large-dose group.HE staining revealed large amounts of glycogen in myocardial cells,no visible disordered arrangement of myocardial cell,fracture and dissolution of muscle fibers and nuclear fission in small-and large-dose group.Compared with those in the positive group,DBP,MBP,LVSP,LVDP and t-dp/dtmax markedly increased（P〈0.05 or 〈0.01）,and LVEDP markedly decreased（P〈0.05） in the small-dose group,whereas DBP and t-dp/dtmax markedly increased（P〈0.05 or 〈0.01）,LVDP,LVEDP and ＋dp/dtmax markedly decreased（P〈0.05 or 〈0.01） in the large-dose group.CONCLUSION： Isopropyl 3-（3,4-dihydroxyphenyl）-2-hydroxylpropanoate significantly inhibits high blood pressure,rapid heart rate and cardiac function excitement in chronic hypoxic rats.So it may significantly protect the cardiac function of chronic hypoxic rats.