三角波形低频脉冲磁场对大鼠心肌微血管内皮细胞增殖、迁移和细胞周期的影响

Effects of triangular low-frequency pulsed magnetic fields on proliferation,migration and cell cycle of cardiac microvascular endothelial cells

目的:研究在固定时间和频率下,三角波形的低频脉冲磁场(LF-PMF)对大鼠心肌微血管内皮细胞(CMECs)增殖、迁移以及细胞周期的影响。方法:实验分为4组(对照组,1.0mT组,1.4mT组,1.8mT组)。对照组不加磁场干预;其余各组分别在频率为15 Hz,磁场强度分别为1.0mT、1.4mT和1.8mT,时间为4 h/d,连续照射3 d的条件下,用三角波形作用离体培养的大鼠CMECs。利用MTT法检测细胞增殖情况,transwell法观察细胞迁移状况,流式细胞仪技术检测细胞周期变化。结果:1.4 mT和1.8 mT组磁场促进CMECs增殖的作用差异显著[(0.200±0.043)A值,vs.(0.159±0.037)A值,(0.225±0.042)A值,vs.(0.159±0.037)A值,P<0.05]。迁移能力与对照组相比也有显著提高[(27.20±4.76)个/视野vs.(22.60±4.77)个/视野,(33.80±3.19)个/视野vs.(22.60±4.77)个/视野,P<0.05]。CMECs在细胞周期中的分布发生改变,DNA合成期(S期)和合成后期(G2期)细胞的比例增加。1.0mT组LF-PMF胞对细胞增殖、迁移以及细胞周期的影响均不明显。结论:低频脉冲磁场磁场对CMECs的生物学作用与磁场的强度相关,1.4mT和1.8mT组LF-PMF促进细胞增殖、迁移及使其DNA合成的能力提高。

AIM：To investigate the effects of low-frequency pulsed magnetic fields（LF-PMFs） on the proliferation,cell cycle and migration of cardiac microvascular endothelial cells（CMECs）.METHODS：Cells were randomly divided into four groups（control group,1.0 mT group,1.4 mT group and 1.8 mT group）.CMECs（except for those in the control group） were exposed to triangular low-frequency pulsed magnetic fields（15 Hz） for 4 h every day and cultured for 3 days.RESULTS：After a 3-day intervention,1.4 mT and 1.8 mT magnetic fields were found to have accelerated the proliferation [（0.200±0.043）A vs.（0.159±0.037）A,（0.225±0.042）A,vs.（0.159±0.037）A,P〈0.05] and the migration（27.20±4.76 vs.22.60±4.77;33.80±3.19 vs.22.60±4.77,P〈0.05） of CMECs.The percentage of cells at the S＋G2 phase increased,but no obvious changes were observed in the 1.0 mT group（P〈0.05）.CONCLUSION：LF-PMFs of 1.4 mT and 1.8 mT accelerate proliferation and migration of CMECs.The percentage of cells at the S＋G phase increases.