3种根尖倒充填材料对体外人牙周韧带细胞创伤模型创面愈合的影响

Effects of three root-end filling materials on human periodontal ligament cells in an in vitro wound model

目的:利用牙周细胞体外创伤模型,探讨MTA、银汞合金和IRM对人牙周韧带细胞生长的影响。方法:在培养皿中形成人牙周韧带细胞的融合培养物,以机械方法去除宽3mm的细胞层条带,形成体外创伤模型。在受损培养物中,分别加入以上3种根尖倒充填材料的浸提液,并以含有10%胎牛血清的培养液作为阳性对照组,含有0.1%胎牛血清的培养液作为阴性对照组。继续培养2、6、9d,对每个时间点细胞增殖能力和对创面的覆盖能力进行检测,应用SPSS11.5软件包对数据进行统计学分析。结果:第2天时,细胞增殖能力为MTA组>银汞合金组≈IRM组;第6天和第9天时,细胞覆盖能力为MTA组>银汞合金组>IRM组(P<0.05)。结论:3种材料中,MTA的生物相容性最好,其次是银汞合金,而IRM最差。

PURPOSE： To compare the effects of mineral trioxide aggregate（MTA）,amalgam and intermediate restorative material（IRM） on human periodontal ligament cells（HPDLC） using an in vitro wound healing model.METHODS： Wound was created by making a 3mm in width incision in the cell monolayer across the length of tissue culture plate,then the cell monolayer was treated with media containing MTA,IRM,and amalgam extracts,respectively.Medium supplemented with fetal bovine serum（10%FBS） served as positive control,and that with 0.1% FBS as negative control.The cells were incubated for 2,6 and 9 days,and at each time point,the levels of both proliferation and cellular wound filling were measured across the width of the wound.SPSS 11.5 software package was used for analyze.RESULTS： There was little cellular repopulation of the wound on the day 2.On the day 6 and day 9,the order of response was MTA〉 amalgam 〉IRM（P﹤0.05）.For the cell proliferative activity,the order was MTA〉 amalgam≈IRM on the day 2.CONCLUSION： This study suggests that the degree of cytotoxic effect in ascending order is MTA,amalgam and IRM.