退变椎体周边关节软骨产生碱性磷酸酶与骨赘形成的关系

The relation between alkaline phosphatase produced by degenerated vertebral peripheral articular cartilage and osteophyte formation

目的：研究椎体骨赘形成的机理。方法：通过切除免颈棘上韧带及棘间韧带和分离颈椎后旁两侧肌肉引起动物颈椎力学上的失衡，经３ 个月的时间的发展而造成免颈椎间盘退变模型。用生物化学方法分别测定每个动物颈椎间盘纤维环和髓核、椎体关节软骨、周边关节软骨中碱性磷酸酶活性。结果：颈椎间盘退变动物椎体周边关节软骨中碱性磷酸酶活性明显升高。结论：研究结果在生物化学上支持椎体骨赘来自于周边关节软骨增殖、化生、钙化和骨化的组织学观察。

Aim:To study mechanism of vertebral osteophyte formation.Methods:A model of cervical intervertebral disc degeneration in the rabbit was established through resection of the cervical supraspinous and interspinous ligaments and detachment of the posterior paravertebral muscles from the cervical spine,elicited by this surgical intervention accelerated the process of intervertebral disc degeneration, and when over 3 months period, induced cervical disc degeneration in the rabbit.The activities of alkaline phosphatase of the anulus fibrosus with nucleus pulposus,vertebral articular cartilage and peripheral articular cartilage in each animal cervical spine were assayed with biochemical method respectively.Results:The activity of alkaline phosphatase in the peripheral articular cartilage of degenerated model animal was obviously increased.Conclusions:This result biochemically supports that the vertebral osteophyte arises from proliferating peripheral articular cartilage,which had been observed histologically.