脑脊液14-3-3蛋白对Creutzfeldt-Jakob病的诊断价值

14-3-3 protein of cerebro spinal fluid in diagnosis of creutzfeldt-Jakob disease

目的 探讨脑脊液中 14 3 3蛋白质对Creutzfeldt Jakob病 (CJD)的诊断价值。方法 应用蛋白质印迹WesternBlot方法检测 61例病人的脑脊液中是否存在 14 3 3蛋白质 ,其中 5例为CJD病人 ,18例为伴有痴呆症状的非CJD疾病 ,3 8例为不伴有痴呆症状的神经系统疾病。在检测 2例肯定的CJD病人的不同条件下保存的脑脊液中 14 3 3蛋白质的稳定性。结果 5例CJD病人CSF14 3 3检测均为阳性。 18例伴有痴呆症状的非CJD的CSF检测均为阴性。而且检测结果说明脑脊液 14 3 3蛋白质是非常稳定的蛋白质 ,保存条件不是决定因素。结论 脑脊液 14 3 3蛋白质的检测对临床上高度怀疑的CJD病例有实验室协助确诊的作用。

Objective To make detection of 14 3 3 protein in the cerebrospinal fluid of the patients with Creutzfeldt Jakob disease. Methods Western blotting was used to detect the 14 3 3 protein in the cerebrospinal fluid from 61 patients with Creutzfeldt Jakob disease and some other CNS disease. There were 5 patients with CJD, 18 patients with dementia were CNS diseases, 38 patients without dementia. The CSF samples from two patients with definite Creutzfeldt Jakob disease were used to test the stability of 14 3 3 protein. Both samples were stored under various conditions. Results The test was positive in all patients with Creutzfeldt Jakob disease and nagitive in all patients with dementia if the one patient with dementia who had had strokes within one month before testing was excluded. All the CSF samples tested that were storded under various conditions were clearly protein positive. Conclusion This should not be a screening test for patients without clinical dementia. It was suggested that the 14 3 3 immunoassay of the cerebrospinal fluid can be widely used to establish the premortal diagnosis of Creutzfeldt Jakob disease.