猴急慢性实验性变应性脑脊髓炎的病理研究

The histopathalogical contrast study of acute and chronic experimental allergic encephalomyelitis with cynomolgus monkeys

目的 探讨急、慢性猴 (猕猴类 )实验性变应性脑脊髓炎 (EAE)的病理特点。方法 从EAE猴病程不同阶段进行病理取材 ,制成切片 ,用HE、Bielschowsky改良法、Page法、Holzer法染色及间接免疫荧光法观察CD4、CD8和B淋巴细胞。结果 ( 1)猴急性EAE脑组织炎症反应重 ,分布在小静脉周围形成血管套 ,以CD4淋巴细胞浸入为主。脱髓鞘病灶微小 ,分布弥散 ,轴突改变轻 ,无出血 ,类似急性播散性脑脊髓炎 (ADEM)。 ( 2 )猴慢性EAE脑组织炎症反应轻 ,分布在血管外周 ,以CD8和B细胞为主。脱髓鞘病灶较大 ,界线清晰 ,呈局灶性分布。病变部位轴突变性严重及轴突丧失 ,有胶质斑块形成 ,与慢性多发性硬化 (MS)的活动期病理改变有惊人的相似。结论 支持猴慢性EAE是MS的优秀模型。另外 ,用相同抗原诱导出不同病程的EAE提示ADEM和MS可能是同一疾病的不同病程 ,或可能是同一种病因 ,而致病程不同。

Objective To investigate the histopathalogical features of acute and chronic experimental allergic encephalomyelitis(EAE) with monkeys. Method EAE models with monkeys were established. Plenty of brain tissue blocks were obtained. Light microscopic paraffin sections were made, stained with Haemaloxylin Eosin(HE), Bielschowsky's, Page's and Holzer's staining methods. Immunocytochemistry was perfomed with indirect FITC labelled fluorescent antibodies against CD4, CD8 and B cells. Results The findings demonstrated that(1) in the acute animal group, the inflammantory response was distinguised, especially around the microscopic veins, and the mojority of the inflammantory cells were CD4 lymphocytes, demyelinating plaques were very small, but spread widely. The hemorrhagic and necrotic changes were not found, resembling acute disseminated encephalomyelitis(ADEM); (2)in the chronic animal group, inflammantory infiltration was slight, and most of the inflammantory cells were CD8 and B lyphocytes. demyelinating patches were much bigger than those in the chronic stage, but located around the ventricles and demyelinating plaques, collagen fibres might be present, the axonal changes including swelling and loss were severe. These features were extraordinarily similar to the active plaques of MS. Conclusion It is concluded that chronic EAE monkeys can be established to be excellent models for studying MS. EAE with the monkeys of the different courses induced indicates the ADEM and MS may be the same or simi disease, but only different in the course or that they may result from the same cause, but different in their course.