视神经损伤后睫状神经营养因子的神经保护效应

The nerve protective effect of ciliary neurotrophic factor after cat optic nerve partialy injury

目的 研究睫状神经营养因子（CNTF）不同给药途径对猫视神经不全损伤后的神经保护效应.方法 完成猫视神经不全损伤模型的制作;实验组和空白对照组分别给予静脉、前房、玻璃体内CNTF注射和生理盐水玻璃体注射;术后15 d双侧上丘、外侧膝状体显微注射DiI逆行神经示踪;不同时间点行视觉诱发电位（PVEP）检测;术后30 d原位心脏灌注;视网膜铺片,视网膜神经节细胞计数;取术侧视神经组织,光镜和电镜观察各组视神经形态变化.结果 空白对照组术侧眼PVEPP100较实验各组振幅明显减低,潜伏期明显延长,差异有统计学意义（P＜0.01）;与空白对照组比较,实验组视网膜神经节细胞存活数量明显增加（P＜0.01）,神经结构更加完整.结论 视神经不全损伤后CNTF不同途径给药具有视神经保护效应,但效应存在差异.

Objective To research the nerve protective effect of ciliary neurotrophic factor （CNTF） after cat optic nerve not entire injury.Methods Completes the cat optic nerve not entire damage model.The experimental group gives CNTF by in the vein,the anterior chambe,the intravitreal to inject separately,the control group gives the physiological saline.Operation latter 15 days of two-sided anterior colliculi,lateral geniculate body microinjection DiI retrogradation nerve tracing.The different time spot completes the examination of visual evoked potential （PVEP）.Operation latter 30 days complete the situ heart dabbling;Retina stretched preparation,retina ganglion cell counting.Takes the optic nerve of operation side,the light microscope and the electron microscope observation morphologic change of optic nerve.Result Controlled group PVEP compares the experimental groups oscillation amplitude of P100 to decrease obviously,incubation period of P100 obvious extension,difference remarkable （P ＜ 0.0l ）.Compares with the controlled group,the survival number of retina ganglion cell of experimental groups increases obviously（P＜0.01 ）,the nerve structure is more complete.Conclusion After optic nerve not entire damage,the CNTF different way injection has the optic nerve protective effect,but effect existence difference.