腹腔不同渗透压液体温热化疗对小鼠腹水瘤的防治作用

The Preventive and Therapeutic Effects of Hyperthermic and Hypotonic Solution in Combination with Drugs on Ascitogenous Tumor Cells in the Mice.

探讨温热、化疗药物、低渗液及其联合应用对小鼠腹水瘤细胞生长的防治作用,并探索结合热化疗药物达到最佳疗效的灌注液介质浓度。方法:以昆明小鼠腹水瘤为模型,观察43℃重蒸馏水(DDW)、37℃丝裂霉素(MMC)生理盐水溶液、43℃MMC不同浓度氯化钠溶液和43℃DDW MMC等不同处理方法对干扰腹水瘤细胞生长、杀伤作用及其毒性作用。结果:以上方法均能破坏小鼠腹水瘤细胞,减少瘤细胞数量,抑制腹水产生,延长小鼠生存时间,使S期肿瘤细胞百分率减少,瘤细胞DNA指数(DI)降低(P<0.05),其中以43℃DDW及MMC腹腔内灌注的方法疗效最佳。结论:低渗液结合热化疗药物行腹腔灌注是防治进展期消化道肿瘤患者发生腹膜种植转移的一种有效方法。

To evaluate the preventive and therapeutic effects of different hypotonic solutions and hyperthermia in combination with anti-tumor drugs on ascitogenous tumor cells of the mice and to select the most effective concentration for the perfusion solution. Methods: The model of ascitogenous tumor cells in the KM mice was used The effects of 43℃ double distilled water(DDW), 37℃MMC in normal sali-ne, 43℃ MMC in hypotonic solution and 43℃ MMC in DDW were compared in terms of interfering the growth of ascitogenous tumor cells, killing and inhibiting the free tumor cells in ascites. Resul-ts: Compared with the control group, therapeutics were demonstrated in all the other groups, with a de-creased number of living cells, a slower progression of their ascites, a longer survival of the mice, and a smaller percent age of tumor cells in the S phase and a decreased DNA index(DI)(P<0.05). However, the best results were observed in the mice receiving MMC in 43℃ DDW. Conclusions: Hypotonic and hyper-thermic solution containing chemotherapeutic agent given as an IP perfusion might be a useful choice in the management of peritoneal carcinomatosis in advanced digestive cancer patients.