采用颈总动脉节段性阻断法建立犬动脉内膜增生模型

A Canine Model of Arterial Intimal Hyperplasia by Segmental Clamping of the Common Carotid Artery.

建立一种较易获得、具有稳定性和重复性且接近于临床实际的动脉内膜增生动物模型。方法:16只健康杂种犬随机分成4组,在游离出右侧颈总动脉后,以微型动脉夹在距环状软骨各2.00cm的近、远端分别阻断颈总动脉0、15、30和60分钟,术后4周取标本进行切片观察及计算机图象分析。结果:于阻断15分钟组,阻断段动脉内膜未见明显增生;阻断30分钟和60分钟者阻断段动脉有新内膜形成,且后者增生程度较前者明显,前者的新生内膜中细胞成份较多,而后者则以细胞间质为主。结论:间歇性节段性动脉阻断法可成功地建立动脉内膜增生的动物模型,其操作方法简单,模型稳定,重复性好,且接近于临床实际,值得推广。

To establish a simple, steady and reproducible animal model of arterial intimal hyper-plasia which would be close to the clinical setting. Methods: Sixteen mongrel dogs were randomly div-ided into four groups, the right common carotid artery was mobilized and clamped 2.0 cm proximal and distal to the cricoid cartilage for 0、15、30 and 60 min respectively. Specimens were harvested four weeks later for microscopic examination, the pictures were analyzed by computer. Results: Neointimal formation which was absent in the group of blockage for 15 min, appeared in groups of blockage for 30 and 60 min, and the degree of intimal hyperplasia was more marked in the 60 min group than that of the 30 min group; the neointima was mainly composed of endothelial cells in the 30 min group, and of extracellular matrix in the 60 min group. Conclusions: The animal model of arterial intimal hyper-plasia introduced is simple, steady, reproducible and close to the clinical setting.