乳源性金黄色葡萄球菌肠毒素的检测与基因分型

Detection and serological type identification of enterotoxins in dairy-derived Staphylococcus aureus

通过检验各类乳制品收集了大量乳源性金葡菌菌株,应用酶联荧光免疫分析法对菌株的肠毒素进行检测分析,并进一步使用荧光定量PCR法对肠毒素基因进行分型。结果表明,乳源性金葡菌肠毒素的检出率与基因型分布均呈现一定显著特征,应用酶联荧光免疫分析法与荧光定量PCR法对肠毒素的检测结果总体无显著性差异。

In the present study,we collected dairy-derived S.aureus strains in the inspection of various dairy products,and then examined the enterotoxin production of the isolates using enzyme-linked fluorescent assay（mini-VIDAS）.Further,Real-time PCR analysis was performed to identify 11 of enterotoxin serological types in these strains.The frequency of enterotoxin-positive isolates presented unique characteristics,as well as the distribution of enterotoxin serological types.In addition,there was no significant difference between the test results of enterotoxin in dairy-derived S.aureus using VIDAS assay and Real-time PCR.