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miR-126对小鼠乳腺上皮细胞增殖及泌乳功能的影响 被引量：8

Impact of miR-126 on proliferation and lactation of Mouse mammary epithelial cells
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摘要 应用脂质体转染技术,改变miR-126在小鼠乳腺上皮细胞中的表达量,采用qRT-PCR、HPLC、Western印迹等技术检测miR-126对小鼠乳腺上皮细胞的影响。结果显示,miR-126沉寂后,细胞增殖能力提高,β-酪蛋白表达增加,乳糖质量浓度增加,孕酮受体(PGR)表达增强。研究表明,miR-126可能通过抑制靶蛋白PGR表达,进而抑制乳腺上皮细胞增殖和泌乳量变化。 In this study,liposome transfection techniqueswere used to change the expression of miR-126 in mouse mammary epithelial cells.The effect of miR-126 on mouse mammary epithelial cells was analyzed by qRT-PCR,HPLC,Western blotting.The results showed that,after miR-126 silence cell proliferation ability enhanced,beta casein expression increased,lactose content increased,progesterone receptor（PGR） expression enhanced.The results suggest that miR-126 probably inhibit mammary epithelial cells and lactation by suppressing expression of PGR.
作者 崔巍 王春梅 李庆章 冯丽 丁巍
机构地区 东北农业大学乳品科学教育部重点实验室
出处 《中国乳品工业》 CAS 北大核心 2011年第3期17-19,45,共4页 China Dairy Industry
基金 国家自然科学基金(No.31072103)
关键词 微小RNA MIR-126 孕酮受体(PGR) 基因沉寂 microRNA miR-126 progestrone receptor gene silencing
分类号 Q936 [生物学—微生物学]
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参考文献12

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共引文献35

同被引文献137

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引证文献8

二级引证文献36

中国乳品工业
2011年 第3期

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