植物转基因育种的分析与研究

Research and Analysis of Transgenic Breeding in Plants

自1983年培育出第一株转基因植物——转基因烟草以来,转基因育种研究已取得飞速发展。应用转基因技术培育了一批抗除草剂、抗虫、抗病、抗逆和优质的转基因材料,有些已实现产业化。就近年来转基因研究在转化技术体系、转化效率和筛选鉴定方面作一概述,就热点问题,如如何培育安全型无选择标记转基因植物和提高外源基因稳定性、表达量等进行分析,并就有关问题提出建议。

Research of transgenic plants has made quick development since the first transgenic plant—tobacco was obtained in 1983.A large number of transgenic materials with herbicide tolerance,insect resistance,disease resistance,stress tolerance and good quality have been cultivated.The production of some transgenic plants has realized commercialization.This paper described advances on transgenic plants in recent years for transformation system,transformation efficiency,selection,identification.About concerned issues,including：cultivate maker-free transgenic plants and exogenous gene expression and stability were analyzed,and suggestions on relevant issues were proposed.