硫酸氨基葡萄糖对正常小鼠免疫功能的影响

Effect of glucosamine sulfate on the immunological function of normal mice

目的了解硫酸氨基葡萄糖对正常小鼠免疫功能的影响。方法将ICR雌性小鼠按体重随机分成低、中、高(150、300、900mg/kg bw)3个剂量组和对照组。连续灌胃30天后,进行脾指数、胸腺指数、细胞免疫、体液免疫、NK细胞、单核-巨噬细胞及NK细胞活性的测定。结果高剂量组小鼠的胸腺指数显著高于对照组(P<0.05);中、高剂量组小鼠迟发型超敏反应(DTH)应激能力、血清溶血素水平及抗体生成能力明显高于对照组(P<0.05);高剂量组小鼠的NK细胞的活性显著高于对照组(P<0.05);硫酸氨基葡萄糖对小鼠脾淋巴细胞的增殖转化能力、腹腔巨噬细胞吞噬鸡红细胞的吞噬率、碳廓清吞噬指数均无影响。结论硫酸氨基葡萄糖从细胞免疫功能、体液免疫功能及NK细胞活性方面能增强正常小鼠的免疫功能。

Objective To observe the effects of glucosamine sulfate on the immunity of normal mice.Methods Female ICR mice were divided by weight into four groups,including low dose group（150mg.kg-1）,medium dose group（300mg.kg-1）,high dose group（900mg.kg-1） and normal control group.Spleen and thymus were weighted 30 days later,cell mediated immunity,humoral immunity,phagocytosis of mononuclear phagocyte and the activity of NK cell were assayed.Results The thymus index in high dose group was decreased significantly in compared with control group（P 0.05）.Compared with the control group,the delayed type hypersensitivity,serum hemolysin and the ability of antibody production were improved significantly in glucosamine sulfate groups（P 0.05）.The activity of NK cell in high dose group was higher than that of the control group（P 0.05）.The proliferation of spleen lymphocyte,the rate of phagocytosis and the carbon particle clearance index in all glucosamine sulfate groups were similar to those in the control group.Conclusion The immune function of normal mice could be enhanced by glucosamine sulfate,including cellular immunity,humoral immunity and the activity of NK cell.