鹅绒委陵菜多糖抗肿瘤作用研究

Anticancer Effects of Polysaccharide from Potentilla anserine L.

目的探讨鹅绒委陵菜多糖(PAP)的抗肿瘤作用。方法采用MTT法研究PAP对体外培养人肝癌细胞SMMC-7721的增殖抑制作用;并将S180荷瘤小鼠随机分组,灌胃给予50,100,200 mg·kg-1剂量的PAP,研究PAP对荷瘤小鼠的抑瘤率和免疫功能的影响。结果体外实验结果显示PAP对SMMC-7721细胞的体外增殖有显著抑制作用,400 mg·L-1 PAP组抑制率最高,为43.57%;PAP对人肝癌SMMC-7721细胞的抑制作用呈剂量依赖性,拟合曲线呈线性相关。动物实验结果显示低、中、高3个剂量的PAP对S180荷瘤小鼠的抑瘤率分别为46.41%,52.29%和59.48%,其中环磷酰胺和低剂量的多糖组合抑瘤率与中、高剂量的多糖抑瘤率相当,并显著高于单用环磷酰胺组的抑瘤率;同时PAP能够显著提高T-淋巴细胞和巨噬细胞吞噬率。结论 PAP具有抗肿瘤活性和增强免疫的功能。

OBJECTIVE To study the antitumor effects and immunological function of polysaccharide from Potentilla anserine L.（PAP） in vivo and in vitro. METHODS The SMMC-7721 cells were treated with different concentration of PAP. The effects of PAP on proliferation of human hepatoma SMMC-7721 cell were examined by MTT assay in vitro, and the relative results were fitted with mathematical model. The S180-bearing mice were intragastrically administered with 50, 100, 200 mg·kg-1 PAP, respectively. The inhibition rate of S180 tumor and immunological function of PAP were measured in vivo. RESULTS The effects on proliferation of SMMC-7721 cell were correlated with the concentration of PAP in vitro. The highest inhibition was 43.57% at the dose of 400 mg·L-1 PAP. The effects and PAP concentration were correlated and regressed. The inhibiting tumor rates were 46.41%, 52.29% and 59.48% in different groups treated with 50, 100 and 200 mg·kg-1 in vivo, respectively. The T-lymphocytes and macrophage phagocytic index increased significantly after the effects of PAP in S 180-bearing mice. CONCLUSION The compound PAP had significant activities of antitumor and immunological function.