柑桔黄龙病的鉴定和柯赫氏定理(英文)

"Candidatus Liberibacter Species": Without Koch's Postulates Completed,Can the Bacterium be Considered as the Causal Agent of Citrus Huanglongbing (Yellow Shoot Disease)?

黄龙(梢)病被认为是世界柑橘生产上的毁灭性病害已超过一个世纪,但该病害的病原学至今还没有清晰地建立起来。根据16S rRNA基因序列分析,一组被称为"Candidatus Liberibacter species"的难培养细菌被认为与黄龙病相关。然而,要确定"Ca.Liberibacter spp."是黄龙病病原的柯赫氏定理并没有真正完成。令我们担忧的是,近年来有些文献频频指出"Ca.Liberibacter spp."是黄龙病的病原,其实该细菌与柑橘寄主的直接病理反应还没有完全确定。我们建议,在黄龙病病原学清楚之前,文献报道在这方面需要有准确的阐述。

Huanglongbing（HLB,yellow shoot disease） has been a destructive disease of citrus around the world for over a century,yet the etiology of the disease has not been definitively established.Based mainly on assays for 16S rRNA gene sequences,fastidious bacteria known as ＂Candidatus Liberibacter species＂ have been associated with HLB.We are concerned that some current literature frequently refers to ＂Ca.Liberibac-ter spp.＂ as the causal or etiological agent of HLB.However,Koch＇s postulates,either sensu stricto or modified,have not yet been completely fulfilled to establish that ＂Ca.Liberibacter spp.＂ are the cause of HLB.Direct pathological interactions between the bacteria and citrus host have not been conclusively documented.We suggest there is a need for the literature to be precise on this point until the etiology of HLB is firmly established.