摘要
Identification of tuber rot pathogen on Stachys sieboldii Miq.was carried out with morphological and molecular methods for the first time.Though the observation of morphology and color of the colonies,morphology of macroconidia,microconidia and chlamydospores,it was preliminarily identified as Fusarium oxysporum.The fungus DNA was amplified by PCR with the universal primers of fungal rDNA ITS1 and ITS4,and sequenced.The sequencing results were logged in GenBank to BLAST,and all of the molecular detections and morphologic observations showed that the pathogen was F.oxysporum.
Identification of tuber rot pathogen on Stachys sieboldii Miq.was carried out with morphological and molecular methods for the first time.Though the observation of morphology and color of the colonies,morphology of macroconidia,microconidia and chlamydospores,it was preliminarily identified as Fusarium oxysporum.The fungus DNA was amplified by PCR with the universal primers of fungal rDNA ITS1 and ITS4,and sequenced.The sequencing results were logged in GenBank to BLAST,and all of the molecular detections and morphologic observations showed that the pathogen was F.oxysporum.
出处
《植物病理学报》
CAS
CSCD
北大核心
2011年第2期210-214,共5页
Acta Phytopathologica Sinica
基金
supported by a grant from the Ministry of Science and Technology,Finance,Special Action of Richening Farmer and Strengthening County Research Program(205)
关键词
草石蚕块
茎腐烂病
病原菌
鉴定方法
Stachys sieboldii Miq.
Fusarium oxysporum
tuber rot
ITS
identification
