蜈蚣多糖蛋白复合物抗斑马鱼胚胎血管生成活性研究

Inhibitory Effect of Polysaccharide-protein Complex from Scolopendra Subspinipes Mutilans on Angiogenesis of Zebrafish Embryo

目的研究蜈蚣多糖蛋白复合物(PPC)的抗血管生成活性。方法采用斑马鱼模型测定血管生成活性。结果 PPC浓度(10～60mg/L)依赖性减少斑马鱼胚胎肠下静脉(SIV)长度,抑制率分别为18.5%,31.3%,42.4%;PPC 103、0和60mg/L组血管生长率分别为(86.2±13.3)%(、75.1±15.7)%和(55.3±10.6)%;三种浓度的PPC可使血管内皮生长因子(VEGF)mRNA表达水平呈不同程度的降低,最高抑制率可达55.0%。结论 PPC具有显著的抗血管生成活性,其作用机制与抑制VEGF mRNA表达有关。

Objective To study the antiangiogenic activity of polysaccharide-protein complex（PPC）from Scolopendra subspinipes mutilans. Methods Zebrafish model was used to evaluate vascular development defects of embryo by morphological observation,staining assay and quantitative endogenous alkaline phosphatase assay. Results PPC decreased the length of subintestinal vein（SIV） with inhibition ratio of 18.5%,31.3% and 42.4% at the concentration of 10.0,30.0 and 60.0mg/L,respectively.Treatment with PPC decreased vessel growth rate of zebrafish embryos and the level of mRNA expression of vascular endothelial growth factor（VEGF） in concentration-dependent manner. Conclusion PPC has significant antiangiogenic activity and the effects may be mediated by the inhibition of VEGF.