小麦TaBS1转录因子基因的克隆、原核表达及Western blot检测

Isolation,Prokaryotic Expression and Western Blot of Transcription Factor TaBS1 Gene from Wheat

转录因子在提高植物抗性中起着重要的作用。本实验从小麦中克隆得到一个新的具有DUF822保守域的转录因子基因TaBS1。为进一步研究该转录因子在参与植物胁迫信号调控中的作用以及蛋白本身的结构、功能和活性等,将TaBS1基因构建到原核表达载体pET-28a(+)上。在终浓度为1mmol·L-1IPTG诱导2h的条件下,得到融合蛋白His-TaBS1,并用Western blot确定所表达的蛋白确实为融合蛋白His-TaBS1。

Transcription factors play an important role in improving resistance in plants.A novel transcription factor gene,named TaBS1,was isolated from wheat.In order to investigate the role of TaBS1 in response to stresses and the structure,function and activity of proteins,the TaBS1 gene was constructed into the prokaryotic expression vector pET-28a（＋）.The fusion protein His-TaBS1 was induced to expression using 1 mmol/L of IPTG.The fusion protein His-TaBS1 was detected by Western blot.This study laid a basis for investigation of protein function,activity,and structure of TaBS1.