蛋壳粉制备氨基酸螯合钙工艺优化

Study on preparation technology of calcium amino acid chelate from eggshell power

以禽蛋加工下脚料蛋壳为原料,通过酸水解制备可溶性钙,调节pH后与甘氨酸螯合制备氨基酸螯合钙。其具有良好的化学和生化稳定性,生物学效价高等特点,能达到既补充氨基酸又补充钙的双重功效。结果表明,蛋壳粉酸水解最佳工艺条件为:1.0g蛋壳粉加入10mL2mol/L的盐酸,在50℃下酸水解15min。此条件下,可溶性钙得率为387.0mg/g;氨基酸螯合钙的最佳工艺条件为:可溶性钙和甘氨酸摩尔比为1:6,在pH为4.8,60℃下螯合反应1.5h。此条件下,可溶性钙的螯合率高达89.32%。因此,以蛋壳为原料,经酸水解溶出可溶性钙再与甘氨酸螯合制备氨基酸螯合钙补钙制剂,为禽蛋加工废弃物的综合利用提供新途径。

Taking eggshell which was abandoned in eggs processing as raw material,the soluble calcium was prepared by acid hydrolysis from eggshell.After adjusting pH, it was chelated with glycine to prepare calcium amino acid chelate. The calcium amino acid chelate was stable to chemistry and biochemistry, and with higher biological potency. It had dual effects on supplement both amino acid and calcium. The results showed that optimum acid hydrolysis condition for soluble calcium was as follows：the 1.0g eggshell powder join in t0mL 2mol/L hydrochloride, under 50℃ acid hydrolysis 15min. Under these conditions the content of soluble calcium was 387.0mg/g.The optimum chelate reaction for calcium amino acid chelate were determined through orthogonal test as follows.The soluble calcium and the glycine mass ratio was 1：6,in pH 4.8, under 60℃chelate reaction 1.5h.The chelate ratio was 89.32% under these conditions.Therefore,Taking egg shell as raw material ,the soluble calcium was dissolved by acid hydrolysis and chelated with glycine to prepare calcium amino acid chelate.This provided a new way for the comprehensive utilization of waste produced in eggs processing.