大果沙棘果渣黄酮对HT29肿瘤细胞活性抑制及DNA损伤作用研究

Inhibition and DNA damage of seabuckthorn residue flavonoids on colon HT29 cancer cells

目的:研究大果沙棘果渣黄酮(Seabuckthorn residue flavonoids,SRF)对人结肠癌HT29细胞生长抑制和DNA损伤的作用。方法:利用MTT比色法测定大果沙棘果渣黄酮对HT29结肠癌细胞生长的抑制率;用单细胞凝胶电泳技术(SCGE)观察大果沙棘果渣黄酮对HT29细胞DNA的损伤情况。结果:大果沙棘果渣黄酮在浓度20～200mg/L时对结肠癌细胞有明显的抑制作用,作用72h最大抑制率为81.28%;单细胞凝胶电泳显示大果沙棘果渣黄酮作用细胞48h后可见明显的彗星状拖尾。结论:大果沙棘果渣黄酮可抑制HT29细胞的生长,对其DNA有致损效应。

Objective：To study the inhibition and DNA damage effects of seabuckthorn residue flavonoids（SRF）on colon HT29 cancer cells.Methods..The inhibition of SRF on human colon HT29 cancer cells was measured with MTT colorimetic method.The DNA damage of HT29 cell was studied in vitro by single cell gel electrophoresis（SCGE）. Result：With SRF 20-200mg/L for 72h,the cell inhibition was significant,the optimized inhibition rate was 81.28%. SCGE showed comet-like tails.Conclusion：SRF can inhibit the growth of HT29 cancer cells and damage cells DNA.