甘利欣对大鼠肺纤维化的治疗作用及对血清IL4/IFN-γ浓度的影响

Role of glycyrrhizin in the treatment of pulmonary fibrosis in rats and impact of this agent on serum IL4/IFN-γ level

目的:研究甘利欣对大鼠肺纤维化的治疗作用及对血清IL4/IFN-γ浓度的影响。方法:40只SD大鼠随机分为对照组、模型组、甘草甜素治疗组、地塞米松组。气管内注入博莱霉素造成动物模型28d后,开始每天甘利欣、地塞米松腹腔给药,于43d时(用药15d后)处死,取肺组织行嗜伊红染色观察肺纤维化程度;肺组织匀浆中羟脯氨酸(HYP)含量,血清测IL4、IFN-γ的浓度。结果:甘利欣治疗后,肺纤维化程度、肺组织匀浆中羟脯氨酸(HYP)含量较模型组减轻;与地塞米松组相仿。甘利欣治疗组血清IL4水平比模型组低,血清IFN-γ水平较模型组与地塞米松组相仿。结论:甘利欣可以减轻博莱霉素诱导的大鼠肺纤维化程度,其机制可能与IL4/IFN-γ有关。

Objective To explore the role of glyeyrrhizin in the treatment of pulmonary fibrosis in rats and the effect of this agent on serum IL4/IFN-γ level. Methods 40 rats were randomly divided into control group, model group,glycyrrhizin group,and dexamethison group.28 days after the establishment of the rat model of pulmonary fibrosis by intratracheal injections of bleomycin,the rats in the two treatment groups received intraperitoneal injections of glyeyrrhizin or dexamethison, and then were sacrificed on day 43. Pathological changes in the lungs were measured by HE staining. The hydroxyprollne content in lung tissue homogenate and serum levels of IL4 and IFN-γ were detected. Results As compared with the model group, the degree of pulmonary fibrosis and the hydroxyproline content decreased in glycyrrhizin group. Serum IL4 level was lower in glyeyrrhizin group than in the model group, whereas IFN-γ level was higher; similar to those in dexamethison group. Conclusions Glycyrrhizin can alleviate the degree of pulmonary fibrosis induced by bleomycin in rats, whose maehanism may be associated with a change in levels of IL4 and IFN-γ.