大蒜愈伤组织诱导条件的优化

Optimization of Callus Induction of Garlic

对大蒜愈伤组织诱导条件进行了优化，结果表明：采用带表皮，外植体大小为0．5cm×0．3cm×0．2cm的广州白皮大蒜蒜瓣，在MS＋3，0mg／L2，4－D＋2．2mg／LIBA培养基中可促使大蒜愈伤组织大量发生．广州白皮大蒜与大头蒜品种对愈伤组织诱导无显著差异．大蒜外植体4℃低温保藏18d可降低出愈污染，但对愈伤组织诱导影响不大．

Plant cell and tissue culture of garlic (Allium sativaum L. ) was a potential alternative approach to produce SOD for the demand of medical uses. This paper deal with the optimization of callus induction of garlic, the results were obtained as follow: When the explant size with epidermis was 0.5 cm ×0.3 cm×0.2 cm, more callus could be induced from the Guangzhou white peel garlic in the culture medium of MS + 3.0 mg/L 2,4-D + 2.2 mg/L IBA. There was no distinct difference between the varieties of Guangzhou white peel garlic and big head garlic in callus inducement. Under cold treatrment of both garlic's explants at 4℃ 18 days the inducing contamination would be reduced but it had no significantly influence on the induction of callus.