摘要
对大蒜愈伤组织诱导条件进行了优化,结果表明:采用带表皮,外植体大小为0.5cm×0.3cm×0.2cm的广州白皮大蒜蒜瓣,在MS+3,0mg/L2,4-D+2.2mg/LIBA培养基中可促使大蒜愈伤组织大量发生.广州白皮大蒜与大头蒜品种对愈伤组织诱导无显著差异.大蒜外植体4℃低温保藏18d可降低出愈污染,但对愈伤组织诱导影响不大.
Plant cell and tissue culture of garlic (Allium sativaum L. ) was a potential alternative approach to produce SOD for the demand of medical uses. This paper deal with the optimization of callus induction of garlic, the results were obtained as follow: When the explant size with epidermis was 0.5 cm ×0.3 cm×0.2 cm, more callus could be induced from the Guangzhou white peel garlic in the culture medium of MS + 3.0 mg/L 2,4-D + 2.2 mg/L IBA. There was no distinct difference between the varieties of Guangzhou white peel garlic and big head garlic in callus inducement. Under cold treatrment of both garlic's explants at 4℃ 18 days the inducing contamination would be reduced but it had no significantly influence on the induction of callus.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1999年第4期54-58,共5页
Journal of South China Agricultural University
基金
国家自然科学基金!29676017
关键词
大蒜
愈伤组织
培养基
低温处理
诱导条件
优化
garlic(Allium sativaum L.)
callus
culture medium
explant
treatment of low temperature