用RFLP鉴定普通小麦—纤毛鹅观草二体附加系中外源染色体的同源转化性

Homoeology Identification of the Alien Chromosomes or Their Arms Involved in T. aestivum-R. ciliaris Disomic Addition Lines by RFLP Analysis

用小麦族7个部分同源群的40个RFLP探针对小麦-纤毛鹅观草二体附加系进行分析,在证实了原有细胞学鉴定结果的基础上,又进一步提供了纤毛鹅观草染色体部分同源群的分子证据.即96K025、96K026中添加的一对纤毛鹅观草染色体B属于第2部分同源群;96KO12、96K013中添加的一对染色体E属于第5部分同源群.对以上株系的衍生株系分析结果表明,染色体B、E在后代中可稳定传递.96K030中添加的一对染色体D与染色体B同属第2部分同源群.端二体附加系96K033所添加的纤毛鹅观草染色体B的一条臂,只与第2群短臂探针有杂交信号,从而初步确定其可能与小麦族第2部分同源群短臂同源.而另一条臂则与第2群长臂同源.

Roegneria ciliaris chromosomes or it's arms involved in Triticum aestivum-Roegneria. ciliaris addition lines were successfully identified by means of RFLP analysis with 40 probes. Results confirmed previous cytological identification and determined the homoeologous grouping of the R. ciliaris chromosomes involved in the addition lines. Lines 96K025 and 96K026 are proved to contain an intact pair of R. ciliaris chromosome B, being identified as belonging to homoeologous group-2. The R. c. chromosomes E added to disomic addition lines 96K012 and 96K013 belong to homoeologous group-5. Line 96K030 contains a pair of R. c. chromosome D, identified as also homoeologous to wheat group-2 chromosomes. A pair of chromosome B long arms added to ditelosomic addkion line 96K033 is homoeologous to short arms of wheat group-2, and the short arm of chromosome B belongs to homoeologous group-2 long arm correspondingly. The results proved RFLP analysis a powerful tool for identifying the alien addition lines and clarifying the homoeology of alien R. ciliaris chromosomes or arms involved.