中国部分地区冠耳霉的遗传多样性研究

Genetic diversity of Conidiobolus coronatus from some regions of China

为研究来自我国不同地区冠耳霉菌株的遗传多样性,用RAPD和ISSR分子标记,对来自中国安徽、山东、贵州、山西和陕西5省的131个冠耳霉菌株（Conidiobolus coronatus）进行遗传多样性分析。筛出的4个RAPD引物和3个ISSR引物共扩增出131条带,其中多态性条带为129条,多态性比率为98.4%,遗传相似性系数范围在0.59~0.99之间,说明受试的冠耳霉具有较为丰富的遗传多样性。利用RAPD和ISSR标记的结果,采用UPGMA聚类分析方法可将绝大多数供试材料分为4大类群。根据所划分的类群,发现冠耳霉之间的亲缘关系与地理来源存在一定的相关性。研究表明,RAPD和ISSR标记可用于冠耳霉遗传多样性的研究。

In order to study genetic diversity of Conidiobolus coronatus isolated from different geographical origin in China,genetic diversities of 131 C.coronatus isolates,randomly sampled from 5 Provinces（Anhui,Shandong,Gueizhou,Shanxi and Shanxi）in China,were analyzed using RAPD（random amplified polymorphic DNA） and ISSR（inter-simple sequence repeat）markers.By using 4 selected RAPD and 3 selected ISSR primers,131 DNA bands were amplified,in which 129 bands showed polymorphism with a rate of 98.4%.The coefficient ranges of genetic similarity of the RAPD and ISSR markers were 0.59-0.99,which indicated that the abundant genetic diversities existed among the tested C.coronatus.UPGMA cluster analysis showed that these isolates were divided into 4 groups,and genetic similarities among C.coronatus isolates were partially related with geo-graphical sources.Based on the results,RAPD and ISSR markers could be applied to detecting genetic diversity among C.coronatus isolates.