2型糖尿病患者血清β_2-GPI/ox-LDL复合物水平

Clinical value of level of β_2-glycoprotein I/ox-LDL complex in sera of patients with type 2diabetes mellitus

目的检测分析2型糖尿病(T2DM)患者血清β2-糖蛋白I/氧化低密度脂蛋白(β2-GPI/ox-LDL)复合物水平。方法以抗人β2-GPI抗体为包被抗体、酶标记抗apoB为检测抗体的血清β2-GPI/ox-LDLELISA检测法,对42例T2DM患者和41例年龄、性别匹配的健康对照者检测分析。用ELISA方法检测血清ox-LDL水平,同时对受检者进行常规血脂水平测定。结果 T2DM组β2-GPI/ox-LDL水平显著高于对照组[(0.89±0.15)U/mlvs(0.81±0.09)U/ml,P<0.05],ox-LDL浓度也明显升高[(48.10±60.40)mg/Lvs(27.06±12.25)mg/L,P<0.05]。相关分析显示,β2-GPI/ox-LDL水平与总胆固醇(TC)、LDL-胆固醇(LDL-C)以及ox-LDL呈显著正相关。结论 T2DM患者血清β2-GPI/ox-LDL复合物水平增高,患者高浓度的血清LDL-C、ox-LDL和机体的高氧化应激状态是β2-GPI/ox-LDL升高的主要原因。

Objective To measure and analyze the concentration of serum β2-glycoprotein I/oxidized low density lipoprotein（β2-GPI/ox-LDL） complex in patients with type 2 diabetes mellitus（T2DM）.Methods The concentrations of β2-GPI/ox-LDL complex were examined in 42 patients with T2DM and 41 age/sex-matched healthy controls by ELISA using rabbit anti-human β2-GPI antibody as the capture antibody,and quantitated with anti-apo（B） polyclonal antibody-enzyme conjugate.The concentrations of ox-LDL in serum were examined by ELISA,and the levels of lipids and lipoproteins in serum were determined simultaneously.Results The concentrations of serum β2-GPI/ox-LDL complex in T2DM were significantly higher than those in controls [（0.89±0.15） U/ml vs（0.81±0.09） U/ml,P0.05].Ox-LDL levels were also significantly elevated in the patients with DM compared with the controls [（48.10±60.40） mg/L vs（27.06±12.25） mg/L,P0.05].The concentrations of β2-GPI/ox-LDL positively correlated with total cholesterol,LDL-C and ox-LDL levels in T2DM.Conclusion β2-GPI/ox-LDL complex levels significantly elevated in T2DM patients,which may be caused by oxidative stress as well as the elevated concentration of serum ox-LDL in T2DM.