单胺氧化酶B基因单核苷酸多态性与藏獒对陌生人攻击行为的关联分析

Association between the Tibetan Mastiff Monoamine Oxidase B Gene Single Nucleotide Polymorphism and Stranger-directed Aggressive Behaviour

采用行为测试的方法,测定藏獒和拉布拉多犬在陌生人接近时的攻击行为差异,应用PCR-RFLP技术检测单胺氧化酶B(MAOB)基因第3外显子单核苷酸多态性,分析其在藏獒(陌生人接近时进行攻击)和拉布拉多犬(陌生人接近时无攻击行为)两个品种间基因型频率与基因频率分布差异。结果表明:MAOB基因型频率与基因频率在两个品种中分布差异不显著(P>0.05)。χ2适合性检验显示,藏獒处于Hardy-Weinberg极不平衡状态(P<0.01),拉布拉多犬处于Hardy-Weinberg平衡状态(P>0.05)。藏獒和拉布拉多犬品种间,在陌生人接近时的攻击行为差异与MAOB基因T119C多态性可能无直接关系。

The aggressive behavior in Tibetan mastiffs and Labrador retrievers when approached directly by a stranger was tested by a behavioural test.The polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）was adopted to detect monoamine oxidaes B（MAOB）gene exon 3 polymorphism,and their genotypes and allelic frequency were estimated among Tibetan mastiffs（aggression when a stranger approaches）and Labrador retrievers（non-aggression when a stranger approaches）.These results indicated that genotypes and allelic frequency of the MAOB gene were not significantly different between the two breeds（P〉0.05）.The result of χ^2 showed that the Tibetan mastiff is biased significantly from Hardy-Weinberg equilibrium（P〈0.01）,while the Labrador retriever fits the Hardy-Weinberg equilibrium（P〉0.05）.Thus the behavioral differences seen in Tibetan mastiffs and Labrador retrievers when a stranger approaches may not relate directly to the MAOB gene T199C polymorphism.